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Author Topic: Nerd/Geek Discussions  (Read 7288 times)
QPO
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« on: April 13, 2009, 08:21:01 PM »
This is for Swing Waltz, EE and many others who can dissertate (I hope this is the right word) here as much as they like  Grin
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SwingWaltz
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« Reply #1 on: April 13, 2009, 08:59:47 PM »
Haha thank you! I'm sure this thread will get warmed up pretty soon!
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elisedance
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ee
« Reply #2 on: April 13, 2009, 09:49:47 PM »
So who won - the Nerds or the Geeks?
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If you must leave the house, go build a home...

The limit of your love is also the limit of your art...
SwingWaltz
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« Reply #3 on: April 13, 2009, 09:50:33 PM »
LoL I thought they are friendly towards eachother.  Roll Eyes
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Dora-Satya Veda
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« Reply #4 on: April 13, 2009, 10:18:19 PM »
really looking forward to this one Smiley

Dora-Satya Veda
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"As we understand more things, everthing is becoming simpler"

Edward Teller
SwingWaltz
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« Reply #5 on: April 14, 2009, 01:43:16 AM »
Why is my substrate phosphorylated without the kinase!!! WHY WHY WHY??? Huh Huh
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Dora-Satya Veda
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« Reply #6 on: April 14, 2009, 01:59:44 AM »
Quote from: SwingWaltz on April 14, 2009, 01:43:16 AM
Why is my substrate phosphorylated without the kinase!!! WHY WHY WHY??? Huh Huh

Sounds like you need to see a doctor......hello is there a doctor on PDO......hellooo.....a doctor is needed over here.



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"As we understand more things, everthing is becoming simpler"

Edward Teller
QPO
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« Reply #7 on: April 14, 2009, 02:00:42 AM »
any doctor will do :-)
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malakawa
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« Reply #8 on: April 14, 2009, 02:38:53 AM »
Quote from: SwingWaltz on April 14, 2009, 01:43:16 AM
Why is my substrate phosphorylated without the kinase!!! WHY WHY WHY??? Huh Huh

go and take a dance lesson. give yourself a break.  Grin
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SwingWaltz
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« Reply #9 on: April 14, 2009, 04:06:23 AM »
Quote from: malakawa on April 14, 2009, 02:38:53 AM
go and take a dance lesson. give yourself a break.  Grin

Will do!  Grin
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elisedance
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ee
« Reply #10 on: April 14, 2009, 04:38:37 AM »
Quote from: SwingWaltz on April 14, 2009, 01:43:16 AM
Why is my substrate phosphorylated without the kinase!!! WHY WHY WHY??? Huh Huh

Obviously your substrate is not a good follower.  One should never phosphorylate until the kinase leads.  Sheesh, send her back to basics...

I'd guess you did not clean up your substrate sufficiently.  Can you attach it for me to take a look? Tongue  Is this a standard reaction or a new one you havn't looked at before?
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If you must leave the house, go build a home...

The limit of your love is also the limit of your art...
SwingWaltz
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« Reply #11 on: April 14, 2009, 04:45:10 AM »
I used purified stathmin as substrate and purified Jun N-terminal Kinase as kinase (GST fusion protein from BL21 cell). A cold in-vitro kinase assay was performed and detection by phospho(ser38)stathmin antibody.

We've done the system in-vivo, but not in vitro. My next step is to do site directed mutagenesis on stathmin, altering the kinase interacting motif,  and see if still gets phosphorylated.

Very naughty follow!!! 
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elisedance
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ee
« Reply #12 on: April 14, 2009, 04:48:41 AM »
then I'm afraid I can't help.  we don't actually do any kinase experiments - well, not as kinases anyway. 

OTOH I might be able to use some stathmin Cheesy we are interested in microtubule activity in nerve terminals - stathmin could be a player...
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If you must leave the house, go build a home...

The limit of your love is also the limit of your art...
SwingWaltz
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« Reply #13 on: April 14, 2009, 04:51:19 AM »
Indeed it does....

I'm looking at stathmin degredation under oxidative stress, and the prevention of degredation by N-acetyl-cysteine.

Now we're talking geek stuff!  Grin
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QPO
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« Reply #14 on: April 14, 2009, 05:13:40 AM »
I am so glad you are happy here.. I will check to see if you are all still alive in a few days.... Lips sealed
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