partnerdanceonline.com
Welcome, Guest. Please login or register.
November 23, 2014, 09:49:39 PM

Login with username, password and session length
Search:     Advanced search
A lot of people are visiting Smiley Smiley
Undecided Undecided but not many are posting....
please say hi Cheesy
116489 Posts in 1857 Topics by 223 Members
Latest Member: dancewithmetoronto
* Home Help Search Calendar Login Register
+  partnerdanceonline.com
|-+  Whats on your mind?
| |-+  Pastimes & Chatter...
| | |-+  Just to chat... (Moderators: QPO, Rugby, Lioness)
| | | |-+  Nerd/Geek Discussions
0 Members and 1 Guest are viewing this topic. « previous next »
Pages: [1] 2 3 ... 13 Print
Author Topic: Nerd/Geek Discussions  (Read 9462 times)
QPO
Moderator
Continental Champion
****
Posts: 20827


Adelaide South Australia


« on: April 13, 2009, 08:21:01 PM »

This is for Swing Waltz, EE and many others who can dissertate (I hope this is the right word) here as much as they like  Grin
Logged

Dance is a delicate balance between perfection and beauty.  ~Author Unknown
Dance Forum
SwingWaltz
Gold Star
***
Posts: 5772


« Reply #1 on: April 13, 2009, 08:59:47 PM »

Haha thank you! I'm sure this thread will get warmed up pretty soon!
Logged
elisedance
Administrator
Blackpool Finalist
*****
Posts: 35026


ee


« Reply #2 on: April 13, 2009, 09:49:47 PM »

So who won - the Nerds or the Geeks?
Logged

If you must leave the house, go build a home...

The limit of your love is also the limit of your art...
SwingWaltz
Gold Star
***
Posts: 5772


« Reply #3 on: April 13, 2009, 09:50:33 PM »

LoL I thought they are friendly towards eachother.  Roll Eyes
Logged
Dora-Satya Veda
Gold Star
***
Posts: 6871


« Reply #4 on: April 13, 2009, 10:18:19 PM »

really looking forward to this one Smiley

Dora-Satya Veda
Logged

"As we understand more things, everthing is becoming simpler"

Edward Teller
SwingWaltz
Gold Star
***
Posts: 5772


« Reply #5 on: April 14, 2009, 01:43:16 AM »

Why is my substrate phosphorylated without the kinase!!! WHY WHY WHY??? Huh Huh
Logged
Dora-Satya Veda
Gold Star
***
Posts: 6871


« Reply #6 on: April 14, 2009, 01:59:44 AM »

Why is my substrate phosphorylated without the kinase!!! WHY WHY WHY??? Huh Huh

Sounds like you need to see a doctor......hello is there a doctor on PDO......hellooo.....a doctor is needed over here.



Logged

"As we understand more things, everthing is becoming simpler"

Edward Teller
QPO
Moderator
Continental Champion
****
Posts: 20827


Adelaide South Australia


« Reply #7 on: April 14, 2009, 02:00:42 AM »

any doctor will do :-)
Logged

Dance is a delicate balance between perfection and beauty.  ~Author Unknown
Dance Forum
malakawa
Open Bronze
*
Posts: 830



« Reply #8 on: April 14, 2009, 02:38:53 AM »

Why is my substrate phosphorylated without the kinase!!! WHY WHY WHY??? Huh Huh

go and take a dance lesson. give yourself a break.  Grin
Logged

Ginger Rogers did everything Fred Astaire did, but she did it backwards and in high heels.

It takes an athlete to dance, but an artist to be a dancer.
SwingWaltz
Gold Star
***
Posts: 5772


« Reply #9 on: April 14, 2009, 04:06:23 AM »

go and take a dance lesson. give yourself a break.  Grin

Will do!  Grin
Logged
elisedance
Administrator
Blackpool Finalist
*****
Posts: 35026


ee


« Reply #10 on: April 14, 2009, 04:38:37 AM »

Why is my substrate phosphorylated without the kinase!!! WHY WHY WHY??? Huh Huh

Obviously your substrate is not a good follower.  One should never phosphorylate until the kinase leads.  Sheesh, send her back to basics...

I'd guess you did not clean up your substrate sufficiently.  Can you attach it for me to take a look? Tongue  Is this a standard reaction or a new one you havn't looked at before?
Logged

If you must leave the house, go build a home...

The limit of your love is also the limit of your art...
SwingWaltz
Gold Star
***
Posts: 5772


« Reply #11 on: April 14, 2009, 04:45:10 AM »

I used purified stathmin as substrate and purified Jun N-terminal Kinase as kinase (GST fusion protein from BL21 cell). A cold in-vitro kinase assay was performed and detection by phospho(ser38)stathmin antibody.

We've done the system in-vivo, but not in vitro. My next step is to do site directed mutagenesis on stathmin, altering the kinase interacting motif,  and see if still gets phosphorylated.

Very naughty follow!!! 
Logged
elisedance
Administrator
Blackpool Finalist
*****
Posts: 35026


ee


« Reply #12 on: April 14, 2009, 04:48:41 AM »

then I'm afraid I can't help.  we don't actually do any kinase experiments - well, not as kinases anyway. 

OTOH I might be able to use some stathmin Cheesy we are interested in microtubule activity in nerve terminals - stathmin could be a player...
Logged

If you must leave the house, go build a home...

The limit of your love is also the limit of your art...
SwingWaltz
Gold Star
***
Posts: 5772


« Reply #13 on: April 14, 2009, 04:51:19 AM »

Indeed it does....

I'm looking at stathmin degredation under oxidative stress, and the prevention of degredation by N-acetyl-cysteine.

Now we're talking geek stuff!  Grin
Logged
QPO
Moderator
Continental Champion
****
Posts: 20827


Adelaide South Australia


« Reply #14 on: April 14, 2009, 05:13:40 AM »

I am so glad you are happy here.. I will check to see if you are all still alive in a few days.... Lips sealed
Logged

Dance is a delicate balance between perfection and beauty.  ~Author Unknown
Dance Forum
Pages: [1] 2 3 ... 13 Print 
« previous next »
Jump to:  

Powered by MySQL Powered by PHP Powered by SMF 1.1.11 | SMF © 2006-2008, Simple Machines LLC Valid XHTML 1.0! Valid CSS!